เข้าสู่ระบบ สมัครสมาชิก

double-stranded dna การใช้

ประโยคมือถือ
  • The cyanophage contained a double - stranded dna genome at ca . 36 . 6kb
  • The genetic material of the new virus is arranged in a similar fashion , encoded in circular , double - stranded dna , and the virus ' s five proteins have similarities to the proteins of other polyoma viruses
  • To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
  • Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
  • The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
  • In this study , the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster , which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226